CRISPR/Cas-based technologies hold great promise as genome editing tools in many genetic diseases, including genodermatoses. However, the clinical translation of genome editing, especially when applied in vivo, remains a big challenge. Aim of this project is the development of highly efficient and easily applicable in vivo treatment options for skin disorders and employ here recessive dystrophic epidermolysis bullosa (RDEB), associated with severe skin blistering and a high mortality rate due to cancer development, as our disease model. Rationally designed CRISPR/Cas9 molecules, previously shown to be highly efficient in restoring collagen type VII expression via COL7A1 reframing, will be delivered into the skin via two application methods: laser microporation and gene gun bombardment. Both delivery approaches represent minimally-invasive treatment options for the local therapy of genodermatoses in general. Efficacy of CRISPR/Cas9-mediated in vivo COL7A1 editing at genomic DNA, RNA, protein and suprastructural level, will disclose the in vivo potential of these technologies for the treatment of genodermatoses.
When I’m not at the lab, I love to spend my free time relaxing at the river reading a good book. Or working up a sweat with an intense yoga session at the park or gym. Always seeking food, beauty and culture in both cities and nature. I want nothing more to portray the aesthetics of simple things through paint or photography. Simple things like a fresh tuna head or a newly sprouted onion. My favorite laboratory occupation is a meditative pipetting of 96-well plate.
Curriculum Vitae Evgeniia Korotchenko
Postdoc at the EB House Austria
Doctoral studies: Molecular Biology, University of Salzburg, Austria
Subject of doctoral thesis: "A novel nanoparticle-based vaccination platform for allergen specific immunotherapy"
Supervisor: Richard Weiss
Master’s studies: Biotechnology, Skolkovo Institute of Science and Technology, Moscow, Russia.
Subject of master’s thesis: "Optimization of the hepatocyte-specific siRNA-conjugates screening system"
Supervisor: Victor Koteliansky
Bachelor’s studies: Engineering Physics, Saint Petersburg Polytechnic University, Russia
Subject of bachelor’s thesis: "Comparative evaluation of impact of non-steroid anti-inflammatory drugs to functional activity of cells of innate immune system"
Supervisor: Marina Makarova
Awards and Fellowships
2019 Poster prize at junior member assembly EAACI, Lissabon, Portugal
2019 ÖGMBT travel grant for EAACI annual congress, Lissabon, Portugal
2019 ÖGAI travel grant for AAI annual congress Immunology, San Diego, USA
2014-2016 Skoltech Scolarship for master’s studies